A New Fluorescent Method to Detect Sulfamidase Activity in Blood, Tissue Extracts and Dried Blood Spots

A New Fluorescent Method to Detect Sulfamidase Activity in Blood, Tissue Extracts and Dried Blood Spots

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Abstract Mucopolysaccharidosis type IIIA (MPS IIIA) is a lysosomal storage disorder due to the taylor te400 deficient activity of sulfamidase (SGSH).Traditionally, measurement of this enzymatic activity has been performed using a fluorescently (4-MU) labeled glycoside substrate.While this substrate is inexpensive and readily available, the current method requires a 2-step procedure that is performed over 2 days.Here we report a new and simplified procedure using the 4-MU substrate.Major advantages of this assay method over the existing fluorescent method include a single step vs.

2-step procedure, an incubation time of 1 hour, and high sensitivity.The reaction is also run on UPLC equipment, which is available in most research labs and permits separation of the endogenous, autofluorescent material from the 4-MU signal.This assay method was developed using the MPS IIIA mouse model, and was validated using mouse plasma, liver and brain extracts, and olea europaea montra dried blood spots.Human MPS IIIA skin fibroblasts and dried blood spots also were used to validate the method.